Journal: Purinergic Signalling

Article Title: Adenosine enhances cisplatin sensitivity in human ovarian cancer cells

doi: 10.1007/s11302-018-9622-7

Figure Lengend Snippet: Expression and functional activity of adenosine receptors in A2780, A2780CisR, and HEY cells. a Gene expression of adenosine receptors using RT-PCR. b Protein expression of adenosine receptors using Western blot. c Concentration-dependent effect of adenosine on cAMP-related luminescence in A2780 cells. d–f Concentration-dependent effect of SLV320, PSB603, and SCH 442416 (selective A1, A2B, and A2A antagonists, respectively) on 200 μM adenosine-induced luminescence in A2780 cells

Article Snippet: Rabbit anti-A1, A2A, A2B, and A3 adenosine receptor antibodies were obtained from Alomone labs (Israel).

Techniques: Expressing, Functional Assay, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Concentration Assay

Journal: Biology Direct

Article Title: OTUD5 promotes the growth of hepatocellular carcinoma by deubiquitinating and stabilizing SLC38A1

doi: 10.1186/s13062-024-00475-0

Figure Lengend Snippet: OTUD5 binds to SLC38A1. ( A ) Label-free mass spectrometry was performed on HCCLM3 cells with or without OTUD5 knockdown to identify differentially expressed proteins. ( B ) The binding between endogenous OTUD5 and SLC38A1 in HCCLM3 cells was demonstrated by co-IP. ( C ) The flag-tagged OTUD5 plasmid was transduced into HEK293T cells. Proteins were precipitated with a FLAG-tagged antibody, and SLC38A1 was detected by Western blotting. ( D ) HCCLM3 and Huh-7 cells that were transduced with OTUD5 shRNAs or NT shRNA were subjected to Western blotting for SLC38A1 and OTUD5 expression. ( E ) HepG2 and Hep3B cells that were transduced with an empty vector (EV) or an OTUD5 expression vector were subjected to Western blotting for SLC38A1 and OTUD5 expression. * P < 0.05

Article Snippet: An anti-OTUD5 rabbit antibody (#20,087, Cell Signaling Technology) was used for immunoprecipitation, and an anti-SLC38A1 mouse antibody (sc-137,032, Santa Cruz Biotechnology, Dallas, Texas, USA) was used for immunoblotting.

Techniques: Mass Spectrometry, Knockdown, Binding Assay, Co-Immunoprecipitation Assay, Plasmid Preparation, Western Blot, Transduction, shRNA, Expressing

Journal: Biology Direct

Article Title: OTUD5 promotes the growth of hepatocellular carcinoma by deubiquitinating and stabilizing SLC38A1

doi: 10.1186/s13062-024-00475-0

Figure Lengend Snippet: OTUD5 deubiquitinates and stabilizes SLC38A1 in HCC cells. ( A ) HCCLM3 cells with OTUD5 knockdown were treated with or without MG132. The downregulation of SLC38A1 protein induced by OTUD5 knockdown was reversed by MG132. ( B and C ) OTUD5 knockdown shortened and OTUD5 overexpression prolonged the half-life of the SLC38A1 protein in HCC cells. ( D and E ) OTUD5 knockdown promoted but OTUD5 overexpression suppressed the ubiquitination of OTUD5 in HCC cells. * P < 0.05

Article Snippet: An anti-OTUD5 rabbit antibody (#20,087, Cell Signaling Technology) was used for immunoprecipitation, and an anti-SLC38A1 mouse antibody (sc-137,032, Santa Cruz Biotechnology, Dallas, Texas, USA) was used for immunoblotting.

Techniques: Knockdown, Over Expression, Ubiquitin Proteomics

Journal: Biology Direct

Article Title: OTUD5 promotes the growth of hepatocellular carcinoma by deubiquitinating and stabilizing SLC38A1

doi: 10.1186/s13062-024-00475-0

Figure Lengend Snippet: SLC38A1 knockdown abolishes OTUD5-enhanced HCC cell proliferation. ( A ) Hep3B and HepG2 cells that were transduced with the corresponding vectors were subjected to Western blotting for SLC38A1 expression. ( B and C ) OTUD5 overexpression led to enhanced HCC cell proliferation, which was strongly reversed by SLC38A1 knockdown. * P < 0.05

Article Snippet: An anti-OTUD5 rabbit antibody (#20,087, Cell Signaling Technology) was used for immunoprecipitation, and an anti-SLC38A1 mouse antibody (sc-137,032, Santa Cruz Biotechnology, Dallas, Texas, USA) was used for immunoblotting.

Techniques: Knockdown, Transduction, Western Blot, Expressing, Over Expression

Journal: Cell metabolism

Article Title: Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance

doi: 10.1016/j.cmet.2020.07.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-SLC38A1 (rabbit polyclonal) , ThermoFisher Scientific , Cat#: PA5–42420 RRID: AB_2605455.

Techniques: Recombinant, Labeling, RNA Sequencing, shRNA, Software